Vacancy: Research Associate – Downstream Processing (fixed term contract – 6 month

Fascinated by protein science and cheese? The Downstream Processing team at Those Vegan Cowboys is responsible for the purification and characterization of native and recombinantly produced milk proteins. We develop DSP processes, study protein formulations and use our purified proteins in functional studies and cheese making.

We are looking for a Research Associate with an interest in protein purification. A great opportunity to work in a dynamic environment, where you can dive deeper into the science behind protein purification through internal training, a springboard for your career. You are available from June-July until December 2022.

The successful candidate should be a creative and motivated person that enjoys challenges with the aim to provide high quality material for characterization and cheese making. The team closely interacts with other disciplines within the company (e.g. Molecular Biology and Fermentation).

 

Job description:

  • Perform protein purifications, using different types of column chromatography (ion exchange, HIC, gel filtration ….), membrane filtration, precipitation techniques etc.
  • Perform different filtration, buffer exchange and concentration techniques (e.g. micro-filtration, ultrafiltration, depth filtration)
  • Evaluate each step in the purification process (yield, protein integrity and purity) using different protein analysis tools such as SDS-PAGE, Western Blot, ELISA, analytical HPLC, …
  • Execute the designed experiments in the lab/pilot, followed by data collection, data analysis and data interpretation.
  • Execute all experiments according to the in-house established standard operating procedures.
  • Collaborate with and help colleagues within the team for execution of experiments.
  • Responsible for individual safety and that of your colleagues by following the correct procedures and policies.

 

Your profile:

  • Master degree in Life Sciences or Bachelor degree with possibly relevant working experience.
  • Insights in protein purification and characterization.
  • Hands-on experience with purification systems is a plus.
  • Ability to work independently as well as in team environment.
  • Problem-solving abilities and analytical skills.
  • Flexible within a dynamic environment, able to multi-task.
  • Communication and organizational skills (interpretation and documentation of data, report writing, planning).
  • Available from June-July until December 2022.

 

Are you our new Temporary Downstream Processing team member?

Please drop us a note at postoffice@thosevegancowboys.com

If you have any questions about these vacancies, please contact Belinda Hunziker by mail or phone + 32 (0)9 261 77 00.

 

About Those Vegan Cowboys:

Those Vegan Cowboys are developing new vegan food products with the same taste as traditional dairy products such as cheese. Our cowboys work in our NewMilkLab, within the grounds of Ghent University. Our researchers collaborate with scientists and players from the traditional dairy industry to develop milk proteins through microbial fermentation. In part not new: nowadays most of all hard cheeses are produced with vegan microbial rennet. Now, Those Vegan Cowboys want to make the casein and other dairy proteins vegan by microbial production.

Those Vegan Cowboys expect that it still will take several years to market products on a serious scale. CEO Jaap Korteweg: “We hope to present something tasty on a smaller scale earlier. The upscaling and traditional processing of high-quality dairy copies takes time. But if it succeeds, this project will make a very positive contribution to animal welfare, fair world food distribution, nature, the climate and biodiversity.” Animal husbandry currently occupies 70% of the current agricultural area. Korteweg: “If you use this area for vegetable meat and dairy products, you only need half or less. Now, large areas of nature are being destroyed daily to feed the growing world population and animal life stock. With vegetarian meat and vegan dairy products, we can reverse this development and give large areas back to nature.”

Those Vegan Cowboys regard the development of a “stainless steel cow” as a logical development within the dairy industry which historically already evolved from hand milking, via the milking machine to the fully automatic milking robot of today. Korteweg: “In Ghent, a predecessor of the milking machine was already demonstrated at the agricultural exhibition in 1910. In 1992, a Dutchman, as first farmer in the world, milked all his cows with a milking robot. The modern dairy farm is no more than a factory where machines insert the grass and trucks take out the milk. The cow is an unnecessarily painful part of this industrial process. The Low Countries have a huge tradition of innovation in the dairy industry. For us, this project is no more than a logical next step.

 

Vacancy: food scientist

Will you write history with the development of your cheese from microbially obtained protein? 

We are looking for a Food Scientist with a strong interest and experience in dairy proteins and taking novel ingredients from applied research to commercialization. The successful candidate should be a creative and highly self-motivated person that enjoys challenges.  

 

Job description: 

  • Generate and apply knowledge about the functionality and the characteristics of our microbially produced milk proteins  
  • Use knowledge data for cheese development activities 
  • Collaborate with our protein and cheese experts  
  • Execute the designed experiments in the lab/food kitchen, followed by data collection, data analysis and data interpretation. 
  • Contribute to IP generation 
  • Collaborate with colleagues over the different departments (upstream and downstream processing) 
  • Responsible for individual safety and that of your colleagues by following the correct procedures and policies. 
  • Communicate with internal stakeholders through meetings and clearly written emails, reports and presentations. 

 

Your profile: 

  • Academic degree in Food Sciences or Food (Bio)technology 
  • Preferably hands-on experience with developing novel products and processes in dairy and/or alt-dairy 
  • Experience in cheese manufacturing (traditional and/or vegan) is a plus 
  • An analytical mindset and the ability to translate research data into product development (you know how to bring the lab data into the food kitchen) 
  • A good knowledge of (novel) food ingredients and their functionality (you know all about taste and texture!) 
  • Hands-on experience with texture analysis, rheology, and emulsification techniques is a plus 
  • Passionate about food, sustainability and health 
  • Ability to work independently with a team player attitude 
  • Flexible within a dynamic environment, able to multi-task. 
  • Communication and organizational skills (interpretation and documentation of data, report writing, planning). 

 

Why join: 

Working as a Vegan Cowboy family member (cheesy but true), means excellent career perspectives in a rapidly growing field, your very own cowboy name (which yes, will be used) and proud ownership of a share in the company through our personnel participation program. Shared with your colleagues, you will own 20% to be exact. 

 

Are you our new Food Scientist team member? 

Please drop us a note at postoffice@thosevegancowboys.com 

If you have any questions about these vacancies, please contact Belinda Hunziker by mail or phone + 32 (0)9 261 77 00. 

Vacancy: Research Associate – Downstream Processing

THIS VACANCY IS CLOSED

 

Fascinated by protein science and cheese? The Downstream Processing team at Those Vegan Cowboys is responsible for the purification and characterization of native and recombinantly produced milk proteins. We develop DSP processes, study protein formulation and use our purified proteins in functional studies and cheese making. We are looking for a Research Associate with a strong interest and experience in protein purification and analysis. The successful candidate should be a creative and highly self-motivated person that enjoys challenges in protein purification with the aim to provide high quality material for characterization and cheese making. The team closely interacts with other disciplines within the company (e.g. Molecular Biology and Fermentation).

Job description:

  • Perform protein purifications, using different types of column chromatography (ion exchange, HIC, gel filtration ….), membrane filtration, precipitation techniques etc.
  • Perform different filtration, buffer exchange and concentration techniques (e.g. micro-filtration, ultrafiltration, depth filtration)
  • Evaluate each step in the purification process (yield, protein integrity and purity) using different protein analysis tools such as SDS-PAGE, Western Blot, ELISA, analytical HPLC, …
  • Assist in the development of new analytical tools to support all teams
  • Execute the designed experiments in the lab/pilot, followed by data collection, data analysis and data interpretation.
  • Execute all experiments according to the in-house established standard operating procedures.
  • Participate in revision of existing and writing of new standard operating procedures.
  • Collaborate with and help colleagues within the team for execution of experiments.
  • Responsible for individual safety and that of your colleagues by following the correct procedures and policies.
  • Communicate with internal stakeholders through meetings and clearly written emails, reports and presentations.

 

Your profile:

  • Master degree in Life Sciences or Bachelor degree with relevant working experience
  • High-level insights in protein purification and characterization.
  • Hands-on experience with purification systems is highly recommended.
  • Experience with analytical HPLC is a plus, including protein analysis e.g. via SEC, RP, ion exchange, …
  • Ability to work independently as well as in team environment.
  • Problem-solving abilities and analytical skills.
  • Flexible within a dynamic environment, able to multi-task.
  • Communication and organizational skills (interpretation and documentation of data, report writing, planning).

 

Why join:

Working as a Vegan Cowboy family member (cheesy but true), means excellent career perspectives in a rapidly growing field, your very own cowboy name (which yes, will be used) and proud ownership of a share in the company through our personnel participation program. Shared with your colleagues, you will own 20% to be exact.

 

Are you our new Downstream Processing team member?

Please drop us a note at postoffice@thosevegancowboys.com

Vacancy: Molecular Biology – Strain Engineering

THIS VACANCY IS CLOSED

 

The Molecular Biology team is looking for a Research Associate with experience in recombinant DNA technology and microbial strain engineering. We are specifically looking for an energetic, creative, and highly self-motivated person that enjoys challenges in microbial strain development with the purpose of heterologous protein expression.

The successful candidate will interact closely with his/her team members and with other disciplines within the company (e.g. the Fermentation team and the Downstream Processing team). Dynamic and pleasant environment where we support each other and work closely together with various people/research groups in various parts of the world.

 

Job description:

• In silico design of DNA/plasmid constructs and execution of the lab-based recombinant DNA work (standard restriction enzyme cloning, Gibson cloning, Golden Gate cloning, …)

• Strain engineering/transformation of yeast, filamentous fungi, bacterial systems.

• Evaluate generated strains via standard techniques such as PCR and (RT)-qPCR analysis, Southern blot, …

• Evaluate protein expression potential of engineered strains via small-scale cultivation, followed by protein detection methods such as gel-based separation (e.g. SDS-PAGE), Western blot, ELISA, …

• Execute the designed experiments in the lab, followed by data collection, data analysis and data interpretation.

• Execute all experiments according to the in-house established standard operating procedures.

• Participate in revision of existing and writing of new standard operating procedures.

• Collaborate with and help colleagues within the team for execution of experiments.

• Responsible for individual safety and that of your colleagues by following the correct procedures and policies.

• Communicate with internal stakeholders through meetings and clearly written emails, reports and presentations.

 

Your profile:

• Bachelor/Master degree in Life Sciences, preferably with relevant work experience.

• Good insights in recombinant DNA technology, microbiology, microbial strain engineering, strain cultivation and analysis, protein analysis.

• Knowledge of vector cloning software (e.g. VectorNTI, Geneious) is a plus.

• Experience with heterologous protein expression in microbial systems is a plus.

• Experience with (or interest in) bioreactor cultivation of micro-organisms (yeast, fungi, bacteria) is an added value.

• Demonstrate ability to work independently as well as in team environment.

• Demonstrate problem-solving abilities and analytical skills.

• Flexible within a dynamic environment, able to multi-task.

• Communication and organizational skills (interpretation and documentation of data, report writing, planning).

 

Are you our new Molecular Biology team member? Please drop us a note at postoffice@thosevegancowboys.com (in Dutch or English). If you have any questions about these vacancies, please contact Belinda Hunziker by mail or phone + 32 (0)9 261 77 00.

Vacancy: Research Associate – Fermentation

THIS VACANCY IS CLOSED

 

For the expansion of the Fermentation team, Those Vegan Cowboys is looking for a Research Associate, who will focus on microbial fermentation process development and optimization to produce recombinant proteins. We are specifically looking for an energetic, creative, and highly self-motivated individual that enjoys challenges in fermentation development. He/she will interact closely with the other disciplines in the group (e.g. Molecular Biology and Downstream Processing).

 

Job description:

  • Conduct fermentation research using shake flasks and bioreactors to improve overall yield of several recombinant proteins of various yeast and fungal systems (in divers bioreactor systems: Sartorius Bplus, and C-DCU, DASGIP and Infors).
  • Perform product recovery experiments using various unit operations.
  • Sample collection and analysis: Biomass determination and protein analysis using SDS-PAGE, Western blot and Bradford assay.
  • Communicate through team meeting presentations and written reports.
  • Maintain lab equipment and bioreactors.
  • Maintain Electronic lab notebooks, write reports and SOPs.

 

Your profile:

  • Master degree, or Bachelor with relevant working experience.
  • Experience with microbial fermentation; experience with fungal expression systems is a plus.
  • Hands-on experience with lab scale fermenter systems (DASGIP, Sartorius Bplus, Sartorius C-DCU fermenters).
  • Demonstrated ability to work independently as well as in team environment, multitask and ensure flexibility in a dynamic environment.
  • Good practical and experimental skills.
  • Hands-on personality with a curious and pro-active mindset.
  • Communication and organizational skills (interpretation and documentation of data, report writing, planning).

 

We offer an interesting job in a dynamic and pleasant work environment in a field with a lot of future. Are you our new Research Associate Fermentation?

Please drop us a note at postoffice@thosevegancowboys.com (in English).

If you have any questions about these vacancies, please contact Belinda Hunziker by mail or phone + 32 (0)9 261 77 00.

Vacancy: Bioprocess Engineer – Downstream Processing

THIS VANACANY IS CLOSED

 

Fascinated by protein science and cheese? The Downstream Processing team at Those Vegan Cowboys is responsible for the purification and characterization of native and recombinantly produced milk proteins. We develop lab scale and pilot scale DSP processes, study protein formulation and use our purified proteins in functional studies and cheese making. We are looking for a Bioprocess Engineer with a strong interest and experience in protein purification and scale-up. The successful candidate should be a creative and highly self-motivated person that enjoys challenges in protein purification with the aim to provide high quality material for characterization and cheese making. The team closely interacts with other disciplines within the company (e.g. the Molecular Biology and Fermentation teams).

 

Job description:

· Implement results from the downstream research program into process development and optimization of downstream recovery and purification, using a broad range of relevant unit operations and separations technologies.

· Use your engineering skills for troubleshooting, process modelling and design of downstream processes from lab scale to pilot and production scale.

· Own complex projects and individual experiments while also working in collaboration with upstream (USP) and downstream processing (DSP) team members.

· Execute the designed experiments, followed by data collection, data analysis and data interpretation.

· Execute all experiments according to the in-house established standard operating procedures.

· Participate in revision of existing and writing of new standard operating procedures.

· Collaborate with and assist colleagues within the team for execution of experiments.

· Responsible for individual safety and that of your colleagues by following the correct procedures and policies.

· Communicate with internal stakeholders through meetings and clearly written emails, reports and presentations.

 

Your profile:

· Degree in Bio-engineering or related fields.

· Minimum 3 years relevant work experience with hands-on technical experience in development, implementation and optimization of (recombinant) protein recovery, purification, formulation and/or drying processes at various scales (lab scale, pilot, production).

· Demonstrated knowledge in protein purification (resins, membrane types for filtration, screening experiments, translation of research results to process development).

· Demonstrated ability to work independently as well as in team environment.

· Demonstrate problem-solving abilities and analytical skills.

· Flexible within a dynamic environment, able to multi-task.

· Communication and organizational skills (interpretation and documentation of data, report writing, planning).

 

Are you our new Downstream Processing team member?

Please drop us a note at postoffice@thosevegancowboys.com .

If you have any questions about these vacancies, please contact Belinda Hunziker by mail or phone + 32 (0)9 261 77 00